Optimal molecular profiling of tissue and tissue components : Defining the best processing and microdissection methods for biomedical applications

Author

BOVA, G. Steven¹ ; ELTOUM, Isam A² ; KIERNAN, John A³ ; SIEGAL, Gene P² ; FROST, Andra R² ; BEST, Carolyn J. M⁴ ; GILLESPIE, John W⁵ ; SU, Gloria H⁶ ; EMMERT-BUCK, Michael R⁴
[1] Departments of Pathology, Oncology, and Institute of Genetic Medicine, The Johns Hopkins Hospital, PELICAN Laboratory, Carnegie 628, Baltimore, MD 21287, United States
[2] Departments of Pathology, Cell Biology, and Surgery and the UAB Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, United States
[3] Department of Anatomy and Cell Biology, University of Western Ontario, London, Canada
[4] Pathogenetics Unit, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States
[5] Science Applications International Corpporation, National Cancer Institute, Bethesda, MD, United States
[6] Departments of Otolaryngology and Pathology, Columbia University College of Physicians and Surgeons, New York, NY, United States

Source

Molecular biotechnology. 2005, Vol 29, Num 2, pp 119-152, 34 p ; ref : 47 ref

CODEN

MLBOEO

ISSN

1073-6085

Scientific domain

Biochemistry, molecular biology, biophysics; Biotechnology

Publisher

Humana Press, Totowa, NJ

Publication country

United States

Document type

Article

Language

English

Author keyword

DNA analysis RNA analysis Tissue processing cytology laser capture microdissection microdissection molecular profiling phenotype-genotype correlation proteomics tissue fixation tissue staining

Keyword (fr)

Application médicale Coloration échantillon Corrélation DNA Fixation Génotype Laser Méthode Optimisation Phénotype Protéomique RNA Tissu

Keyword (en)

Medical application Staining Correlation DNA Fixation Genotype Laser Method Optimization Phenotype Proteomics RNA Tissue

Keyword (es)

Aplicación medical Coloración muestra Correlación DNA Fijación Genotipo Láser Método Optimización Fenotipo Proteómica RNA Tejido

Classification

Pascal

002 Biological and medical sciences / 002A Fundamental and applied biological sciences. Psychology / 002A31 Biotechnology

Discipline

Biotechnology

Origin

Inist-CNRS

Database

PASCAL

INIST identifier

16597720

Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS