Fluorescence Characterization of Co-immobilization-Induced Multi-Enzyme Aggregation in a Polymer Matrix Using Förster Resonance Energy Transfer (FRET): Toward the Metabolon Biomimic
- Author
- FEI WU1 ; MINTEER, Shelley D1
[1] Department of Chemistry and Materials Science and Engineering, University of Utah, Salt Lake City, Utah 84112, United States - Source
Biomacromolecules. 2013, Vol 14, Num 8, pp 2739-2749, 11 p ; ref : 34 ref
- ISSN
- 1525-7797
- Scientific domain
- Biochemistry, molecular biology, biophysics; Polymers, paint and wood industries
- Publisher
- American Chemical Society, Washington, DC
- Publication country
- United States
- Document type
- Article
- Language
- English
- Keyword (fr)
- Aconitate hydratase Activité enzymatique Agrégation moléculaire Biomimétique Chitosane Coimmobilisation Dérivé du chitosane Enzyme immobilisée Ethylène(tétrafluoro) copolymère Ethylèneimine copolymère Ethylèneimine dérivé copolymère Ethylèneimine polymère Etude expérimentale Malate dehydrogenase Méthode étude Répartition spatiale Spectrométrie fluorescence Sulfonate copolymère Support Synthase Transfert énergie résonnant Chitosane(N-butyl) Citrate synthase Ethylèneimine(N-octyl) copolymère Formation métabolon Interaction enzyme enzyme Carbon-oxygen lyases Enzyme Hydro-lyases Lyases Oxidoreductases
- Keyword (en)
- Aconitate hydratase Enzymatic activity Molecular aggregation Biomimetics Chitosan Coimmobilization Chitosan derivatives Immobilized enzyme Tetrafluoroethylene copolymer Ethyleneimine copolymer Ethyleneimine derivative copolymer Polyethylene imine Experimental study Malate dehydrogenase Investigation method Spatial distribution Fluorescence spectrometry Sulfonate copolymer Support Synthase Resonant energy transfer Carbon-oxygen lyases Enzyme Hydro-lyases Lyases Oxidoreductases
- Keyword (es)
- Aconitate hydratase Actividad enzimática Agregación molecular Quitosano Coinmovilización Quitosano derivado Enzima inmovilizada Etileno(tetrafluoro) copolímero Etilenoimina copolímero Etilenoimina derivado copolímero Etilenoimina polímero Estudio experimental Malate dehydrogenase Método estudio Distribución espacial Espectrometría fluorescencia Sulfonato copolímero Soporte Synthase Transferencia energía resonante Carbon-oxygen lyases Enzima Hydro-lyases Lyases Oxidoreductases
- Classification
- Pascal
- 001 Exact sciences and technology / 001D Applied sciences / 001D09 Physicochemistry of polymers / 001D09D Organic polymers / 001D09D04 Properties and characterization / 001D09D04I Special properties (catalyst, reagent or carrier)
- Pascal
- 002 Biological and medical sciences / 002A Fundamental and applied biological sciences. Psychology / 002A31 Biotechnology / 002A31C Methods. Procedures. Technologies / 002A31C05 Immobilization techniques / 002A31C05B Immobilization of enzymes and other molecules
- Discipline
- Biotechnology Physical chemistry of polymers
- Origin
- Inist-CNRS
- Database
- PASCAL
- INIST identifier
- 27658773
Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS
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DOI
10.1021/bm400569k 
https://dx.doi.org/10.1021/bm400569k
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