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Reduction of the nitro group during sample preparation may cause underestimation of the nitration level in 3-nitrotyrosine immunoblotting

Author
SÖDERLING, Ann-Soft1 ; HULTMAN, Lena2 ; DELBRO, Dick3 4 ; HØJRUP, Peter5 ; CAIDAHI, Kenneth1 6
[1] Sahlgrenska Academy, Department of Clinical Physiology, Sahlgrenska University Hospital, Goteborg, Sweden
[2] Sahlgrenska Academy, Department of Clinical Medicine, Sahlgrenska University Hospital, Göteborg, Sweden
[3] Sahlgrenska Academy, Department of Clinical Surgery, Sahlgrenska University Hospital, Göteborg, Sweden
[4] Department of Pure and Applied Natural Sciences, University of Kalmar, Kalmar, Sweden
[5] Department of Biochemistry and Molecular Biology, University of South Denmark, Odense, Denmark
[6] Karolinska Institutet, Department of Molecular Medicine and Surgery, Clinical Physiology N2:01, Karolinska University Hospital, Stockholm, Sweden
Source

Journal of chromatography. B. 2007, Vol 851, Num 1-2, pp 277-286, 10 p ; ref : 36 ref

ISSN
1570-0232
Scientific domain
Medical laboratory techniques; Pharmacology drugs
Publisher
Elsevier Science, Amsterdam
Publication country
Netherlands
Document type
Article
Language
English
Author keyword
3-Nitrotyrosine Angiotensin II Mass spectrometry Temperature Western blot
Keyword (fr)
Angiotensine II Composé nitro Méthode immunoblotting Nitration Peptide Préparation échantillon Réduction chimique Spectrométrie masse Température Tyrosine(nitro) Hormone peptide Octapeptide Système renin angiotensine
Keyword (en)
Angiotensin II Nitro compound Immunoblotting assay Nitration Peptides Sample preparation Chemical reduction Mass spectrometry Temperature Peptide hormone Octapeptide Renin angiotensin system
Keyword (es)
Angiotensina II Compuesto nitro Western blotting Nitración Péptido Preparación muestreo Reducción química Espectrometría masa Temperatura Hormona péptido Octapéptido Sistema renin angiotensina
Classification
Pascal
002 Biological and medical sciences / 002A Fundamental and applied biological sciences. Psychology / 002A02 Analytical, structural and metabolic biochemistry

Pascal
002 Biological and medical sciences / 002B Medical sciences / 002B02 Pharmacology. Drug treatments / 002B02A General pharmacology / 002B02A02 Analysis

Discipline
Analytical, structural and metabolic biochemistry General pharmacology
Origin
Inist-CNRS
Database
PASCAL
INIST identifier
18772877

Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS

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