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Stabilization of thin-layer agarose gels after isoelectric focusing with polyacrylamide enables reverse imidazole-zinc staining and facilitates two-dimensional gel electrophoresis

Author
HELLMAN, Jukka1
[1] Department of Biochemistry and Food Chemistry/Biotechnology, University of Turku, Tykistökatu 6 A, 20520 Turku, Finland
Source

Analytical and bioanalytical chemistry. 2008, Vol 392, Num 1-2, pp 239-245, 7 p ; ref : 31 ref

ISSNNV
1618-2642
Scientific domain
Food science technology; Analytical chemistry; Chemistry
Publisher
Springer, Heidelberg
Publication country
Germany
Document type
Article
Language
English
Author keyword
Agarose isoelectric focusing GelBond Imidazole-zinc reverse staining Immune complex Two-dimensional electrophoresis
Keyword (fr)
Acrylamide polymère Agarose Alkylation Argent Colorant triarylméthanique Composé du sodium Conformation Digestion Dimension pore Electrophorèse bidimensionnelle Electrophorèse gel Ester polymère Focalisation isoélectrique Imidazole Masse moléculaire Peptide Protéine Sensibilité Spectrométrie masse Sulfate(lauryl) Zinc
Keyword (en)
Acrylamide polymer Agarose Alkylation Silver Triarylmethane dye Sodium compound Conformation Digestion Pore size Two dimensional electrophoresis Gel electrophoresis Ester polymer Isoelectric focusing Imidazole Molecular mass Peptides Protein Sensitivity Mass spectrometry Lauryl sulfate Zinc
Keyword (es)
Acrilamida polímero Agarosa Alquilación Plata Colorante triarilmetánico Sodio compuesto Conformación Digestión Dimensión poro Electrofóresis bidimensional Electroforesis gel Ester polímero Focalización isoeléctrica Masa molecular Péptido Proteína Sensibilidad Espectrometría masa Lauril sulfato Zinc
Classification
Pascal
001 Exact sciences and technology / 001C Chemistry / 001C04 Analytical chemistry / 001C04C Spectrometric and optical methods

Discipline
Analytical chemistry
Origin
Inist-CNRS
Database
PASCAL
INIST identifier
20608517

Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS

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