Probing the role of two hydrophobic active site residues in the human dihydrofolate reductase by site-directed mutagenesis
- Author
- SCHWEITZER, B. I1 ; SRINIVASAN SRIMATKANDADA; GRITSMAN, H; SHERIDAN, R; RENGACHARI VENKATARAGHAVAN; BERTINO, J. R
[1] Memorial Sloan-Kettering cancer cent., New York NY 10021, United States - Source
The Journal of biological chemistry (Print). 1989, Vol 264, Num 34, pp 20786-20795 ; ref : 48 ref
- CODEN
- JBCHA3
- ISSN
- 0021-9258
- Scientific domain
- Biochemistry, molecular biology, biophysics; Biotechnology
- Publisher
- American Society for Biochemistry and Molecular Biology, Bethesda, MD
- Publication country
- United States
- Document type
- Article
- Language
- English
- Keyword (fr)
- Activité enzymatique Cinétique Constante dissociation Fixation ligand Homme Interaction moléculaire Mutagenèse dirigée Recombinaison Structure 3 dimensions Tetrahydrofolate dehydrogenase Vecteur
- Keyword (en)
- Enzymatic activity Kinetics Dissociation constant Ligand binding Human Molecular interaction Site directed mutagenesis Recombination Spatial structure Tetrahydrofolate dehydrogenase Vector
- Keyword (es)
- Actividad enzimática Cinética Constante disociación Fijación ligando Hombre Interacción molecular Mutagénesis dirigida Recombinación Estructura 3 dimensiones Tetrahydrofolate dehydrogenase Vector
- Classification
- Pascal
- 002 Biological and medical sciences / 002A Fundamental and applied biological sciences. Psychology / 002A02 Analytical, structural and metabolic biochemistry / 002A02E Enzymes and enzyme inhibitors / 002A02E02 Oxidoreductases
- Discipline
- Analytical, structural and metabolic biochemistry
- Origin
- Inist-CNRS
- Database
- PASCAL
- INIST identifier
- 6929264
Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS
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DOI
10.1016/S0021-9258(19)47131-7 
https://dx.doi.org/10.1016/S0021-9258(19)47131-7
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